3D Nanofabrication: 3D Nanofabrication of Fluidic Components by Corner Lithography

1. Erwin J. W. Berenschot¹, 
2. Narges Burouni¹,
3. Bart Schurink², 
4. Joost W. van Honschoten¹,
5. Remco G. P. Sanders¹, 
6. Roman Truckenmuller², 
7. Henri V. Jansen¹, 
8. Miko C. Elwenspoek¹, 
9. Aart A. van Apeldoorn²,
10. Niels R. Tas^1,*

Article first published online: 15 DEC 2012

DOI: 10.1002/smll.20129013

A pyramid microarray comprising microsized nanowires made by 3D corner lithography is presented by N. R. Tas and co-workers on page 3823. The arrays are integrated into a cell-seeding device for the entrapment of single cells. Polystyrene microspheres are shown efficiently captured from a suspension of homogeneously shaped spheres. The same procedure is done with a suspension of primary bovine chondrocytes, after which their phenotype is studied over 48 h. The magnified electron micrograph shows the efficient entrapment of 1 cell per pyramid after 2 h of cell culture. Cells maintain their native round morphology during entrapment, while the onset of protein deposition starts between these confined cells.