A Mechanically Controlled Indicator Displacement Assay

1. Dr. Keita Sakakibara^1,2,
2. Leo A. Joyce³,
3. Dr. Taizo Mori^1,2,
4. Takuya Fujisawa¹,
5. Dr. Shagufta H. Shabbir³,
6. Dr. Jonathan P. Hill^1,2,
7. Prof. Eric V. Anslyn^3,*,
8. Prof. Katsuhiko Ariga^1,2,*

Article first published online: 29 AUG 2012

DOI: 10.1002/anie.201203402

 Push a host: Mechanical compression was applied to a host monolayer at an interface, which facilitated an indicator displacement assay. The fluorescence resonance energy transfer (FRET) between the host and indicator was switched on by this compression. Addition of D-glucose caused the indicator to be displaced, effectively quenching the FRET process.