Sunday, August 26, 2012

Shedding Light on Helical Microtubules: Real-Time Observations of Microtubule Self-Assembly by Light Microscopy


Department of Chemical and Biomolecular Engineering,University of Maryland, College Park, Maryland 20742-2111, United States
J. Am. Chem. Soc., Article ASAP
DOI: 10.1021/ja3031856
Publication Date (Web): August 21, 2012
Copyright © 2012 American Chemical Society


Helical tubules are a fascinating and an intriguing class of self-assemblies. They occur frequently in biology and are believed to be intermediates in formation of gallstones. The pathway by which amphiphiles transform from an initial state of vesicles or micelles into such tubules has puzzled soft matter physicists, and it has raised important questions about the interplay between molecular chirality and self-assembly. Here, for the first time, we demonstrate direct, real-time observations by light microscopy of the pathway to helical microtubules from an initial solution of nanoscale vesicles. The tubules are formed in aqueous mixtures of the single-tailed diacetylenic surfactant, 10,12-pentacosadiynoic acid (PCDA), and a short-chain alcohol. The stepwise process involves nucleation of thin helical microribbons from the vesicle solution. These ribbons then thicken, rearrange, and fold into closed tubules. Subsequently, most tubules further rearrange into plate-like structures, and once again, we are able to visualize this process in real time. A notable aspect of the above system is that the precursors are achiral; yet, the tubules are formed from helical ribbons. Our study provides new insights into tubule formation that will be valuable in clarifying and refining theoretical models for these fascinating structures.

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