Direct Visualization of the Movement of a Single T7 RNA Polymerase and Transcription on a DNA Nanostructure†

1. Dr. Masayuki Endo^1,3,*, 
2. Koichi Tatsumi²,
3. Kosuke Terushima², 
4. Yousuke Katsuda²,
5. Kumi Hidaka², 
6. Prof. Yoshie Harada¹, 
7. Prof. Hiroshi Sugiyama^1,2,3,*

Article first published online: 29 JUL 2012

DOI: 10.1002/anie.201201890

Snapshots of transcription: Movement of a single molecule of T7 RNA polymerase (RNAP) along a double-stranded DNA (dsDNA) template attached to a DNA origami platform (green, see scheme) was recorded using high-speed AFM. A one kilobase template dsDNA containing the T7 promoter was used to record AFM images of transcription of the template dsDNA by RNAP and the resulting biotinylated RNA product was detected by streptavidin-labeling.