Force-Controlled Fluidic Injection into Single Cell Nuclei

1. Orane Guillaume-Gentil¹, 
2. Eva Potthoff¹,
3. Dario Ossola², 
4. Pablo Dörig², 
5. Tomaso Zambelli^2,*, 
6. Julia A. Vorholt^1,*

Article first published online: 20 NOV 2012

DOI: 10.1002/smll.201202276

urpassing the physical barriers of the cytoplasmic and nuclear membranes to deliver biomolecules directly into cell nuclei offers opportunities to investigate dynamic processes in living cells. The potential of atomic force microscopy coupled to microfluidics (FluidFM) for volume-controlled intranuclear delivery is demonstrated, whereby minimally invasive microchanneled probes are remotely driven with high spatiotemporal resolution.